Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs.

ABSTRACT:

Background

Process formation by glial cells is crucial to their function. Mayven, an actin binding, multi-domain polypeptide, and member of the BTB-BACK-Kelch family have been shown to be important in oligodendrocyte process extension. To assess the role of Mayven in neural cell process extension we have tracked the subcellular distribution of exogenous Mayven following expression of a rat Mayven -EGFP cDNA in a variety of neural cell backgrounds and specifically in OEC tranfectants following drug treatment to disrupt the integrity of the cytoskeleton. A comparison was made between the subcellular localization following transient transfection of OECs with full-length Mayven cDNA and a series of mutant domain constructs.

Results

The subcellular location of Mayven in OEC trans

SUBMITTER: Montague P 

PROVIDER: S-EPMC2901378 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature