Structural and functional characterization of diffusible signal factor family quorum-sensing signals produced by members of the Burkholderia cepacia complex.

ABSTRACT: Previous work has shown that Burkholderia cenocepacia produces the diffusible signal factor (DSF) family signal cis-2-dodecenoic acid (C(12):Delta(2), also known as BDSF), which is involved in the regulation of virulence. In this study, we determined whether C(12):Delta(2) production is conserved in other members of the Burkholderia cepacia complex (Bcc) by using a combination of high-performance liquid chromatography, mass spectrometry, and bioassays. Our results show that five Bcc species are capable of producing C(12):Delta(2) as a sole DSF family signal, while four species produce not only C(12):Delta(2) but also a new DSF family signal, which was identified as cis,cis-11-methyldodeca-2,5-dienoic acid (11-Me-C(12):Delta(2,5)). In addition, we demonstrate that the quorum-sensing signal

SUBMITTER: Deng Y 

PROVIDER: S-EPMC2901730 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature