Mapping of lysine methylation and acetylation in core histones of Neurospora crassa.

ABSTRACT: Core histones are susceptible to a variety of post-translational modifications (PTMs), among which methylation and acetylation play critical roles in various chromatin-dependent processes. The nature and biological functions of these PTMs have been extensively studied in plants, animals, and yeasts. In contrast, the histone modifications in Neurospora crassa, a convenient model organism for multicellular eukaryotes, remained largely undefined. In this study, we used several mass spectrometric techniques, coupled with HPLC separation and multiple-protease digestion, to identify the methylation and acetylation sites in core histones isolated from Neurospora. Electron transfer dissociation (ETD) was employed to fragment the heavily modified long N-terminal peptides. In addition, accurate mass

SUBMITTER: Xiong L 

PROVIDER: S-EPMC2902163 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature