FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair.

ABSTRACT: In response to DNA damage, the Fanconi anemia (FA) core complex functions as a signaling machine for monoubiquitination of FANCD2 and FANCI. It remains unclear whether this complex can also participate in subsequent DNA repair. We have shown previously that the FANCM constituent of the complex contains a highly conserved helicase domain and an associated ATP-dependent DNA translocase activity. Here we show that FANCM also possesses an ATP-independent binding activity and an ATP-dependent bi-directional branch-point translocation activity on a synthetic four-way junction DNA, which mimics intermediates generated during homologous recombination or at stalled replication forks. Using an siRNA-based complementation system, we found that the ATP-dependent activities of FANCM are required for ce

SUBMITTER: Xue Y 

PROVIDER: S-EPMC2902294 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature