High accuracy, high-resolution prevalence measurement for the majority of locally expressed regulatory genes in early sea urchin development.

ABSTRACT: Accurate measurements of transcript abundance are a prerequisite to understand gene activity in development. Using the NanoString nCounter, an RNA counting device, we measured the prevalence of 172 transcription factors and signaling molecules in early sea urchin development. These measurements show high fidelity over more than five orders of magnitude down to a few transcripts per embryo. Most of the genes included are locally restricted in their spatial expression, and contribute to the divergent regulatory states of cells in the developing embryo. In order to obtain high-resolution expression profiles from fertilization to late gastrulation samples were collected at hourly intervals. The measured time courses agree well with, and substantially extend, prior relative abundance measuremen

SUBMITTER: Materna SC 

PROVIDER: S-EPMC2902461 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature