Glutamate receptors on myelinated spinal cord axons: I. GluR6 kainate receptors.

ABSTRACT:

Objective

The deleterious effects of glutamate excitotoxicity are well described for central nervous system gray matter. Although overactivation of glutamate receptors also contributes to axonal injury, the mechanisms are poorly understood. Our goal was to elucidate the mechanisms of kainate receptor-dependent axonal Ca(2+) deregulation.

Methods

Dorsal column axons were loaded with a Ca(2+) indicator and imaged in vitro using confocal laser-scanning microscopy.

Results

Activation of glutamate receptor 6 (GluR6) kainate receptors promoted a substantial increase in axonal [Ca(2+)]. This Ca(2+) accumulation was due not only to influx from the extracellular space, but a significant component originated from ryanodine-dependent intracellular stores, which, in turn, depende

SUBMITTER: Ouardouz M 

PROVIDER: S-EPMC2902553 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature