Genome-wide analysis of gene expression regulated by the HAT cofactor Trrap in conditional knockout cells.

ABSTRACT: Transactivation/transformation-domain associated protein (TRRAP) is a component of several multi-protein HAT complexes implicated in both transcriptional regulation and DNA repair. We recently identified Trrap, the murine ortholog of TRRAP, as an essential protein implicated in mitotic progression control, although its target genes are not known. In the present study, we analyzed the expression profiles of Trrap-responsive genes, using cDNA microarray in mitotic cells. From a panel of 17 664 transcript elements, we found that loss of Trrap leads to expression alteration of a large fraction of genes at mitotic stage. Functional classification of these genes indicates that Trrap influences a variety of cellular processes including cell cycle progression, cytoskeleton and cell adhesion, prote

SUBMITTER: Herceg Z 

PROVIDER: S-EPMC290270 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature