The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells.

ABSTRACT: Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog species Rana pipiens. The isolated clones were approximately 90% identical to a predicted transposase gene sequence from Xenopus laevis, but contained an unpredicted, approximately 180 bp region encoding the N-terminus of the putative transposase. None of these native genes was found to be active. Therefore, a consensus sequence of the transposase gene was derived. This engineered transposase and the transposon inverted repeats together constitute the components of a novel tran

SUBMITTER: Miskey C 

PROVIDER: S-EPMC290277 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature