Validation of a novel, fully integrated and flexible microarray benchtop facility for gene expression profiling.

ABSTRACT: Here we describe a novel microarray platform that integrates all functions needed to perform any array-based experiment in a compact instrument on the researcher's laboratory benchtop. Oligonucle otide probes are synthesized in situ via a light- activated process within the channels of a three-dimensional microfluidic reaction carrier. Arrays can be designed and produced within hours according to the user's requirements. They are processed in a fully automatic workflow. We have characterized this new platform with regard to dynamic range, discrimination power, reproducibility and accuracy of biological results. The instrument detects sample RNAs present at a frequency of 1:100 000. Detection is quantitative over more than two orders of magnitude. Experiments on four identical arrays with 6

SUBMITTER: Baum M 

PROVIDER: S-EPMC290286 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature