Gene expression profile of epithelial cells and mesenchymal cells derived from limbal explant culture.

ABSTRACT:

Purpose

Limbal stem cell deficiency is a challenging clinical problem and the current treatment involves replenishing the depleted limbal stem cell (LSC) pool by either limbal tissue transplantation or use of cultivated limbal epithelial cells (LEC). Our experience of cultivating the LEC on denuded human amniotic membrane using a feeder cell free method, led to identification of mesenchymal cells of limbus (MC-L), which showed phenotypic resemblance to bone marrow derived mesenchymal stem cells (MSC-BM). To understand the transcriptional profile of these cells, microarray experiments were carried out.

Methods

RNA was isolated from cultured LEC, MC-L and MSC-BM and microarray experiments were carried out by using Agilent chip (4x44 k). The microarray data was validated by usi

SUBMITTER: Polisetti N 

PROVIDER: S-EPMC2903463 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature