Stable morphology, but dynamic internal reorganisation, of interphase human chromosomes in living cells.

ABSTRACT: Despite the distinctive structure of mitotic chromosomes, it has not been possible to visualise individual chromosomes in living interphase cells, where chromosomes spend over 90% of their time. Studies of interphase chromosome structure and dynamics use fluorescence in-situ hybridisation (FISH) on fixed cells, which potentially damages structure and loses dynamic information. We have developed a new methodology, involving photoactivation of labelled histone H3 at mitosis, to visualise individual and specific human chromosomes in living interphase cells. Our data revealed bulk chromosome volume and morphology are established rapidly after mitosis, changing only incrementally after the first hour of G1. This contrasted with the behaviour of specific loci on labelled chromosomes, which showe

SUBMITTER: Muller I 

PROVIDER: S-EPMC2903487 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature