Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.

ABSTRACT:

Background

Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.

Results

Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (

SUBMITTER: Huss S 

PROVIDER: S-EPMC2903497 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature