Overexpression of S100A4 in human cancer cell lines resistant to methotrexate.

ABSTRACT:

Background

Methotrexate is a chemotherapeutic drug that is used in therapy of a wide variety of cancers. The efficiency of treatment with this drug is compromised by the appearance of resistance. Combination treatments of MTX with other drugs that could modulate the expression of genes involved in MTX resistance would be an adequate strategy to prevent the development of this resistance.

Methods

The differential expression pattern between sensitive and MTX-resistant cells was determined by whole human genome microarrays and analyzed with the GeneSpring GX software package. A global comparison of all the studied cell lines was performed in order to find out differentially expressed genes in the majority of the MTX-resistant cells. S100A4 mRNA and protein levels were determine

SUBMITTER: Mencia N 

PROVIDER: S-EPMC2903526 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature