Lipid rafts play an important role for maintenance of embryonic stem cell self-renewal.

ABSTRACT: Lipid rafts are cholesterol-rich microdomains of cell membranes that have a variety of roles in cellular processes including receptor-mediated signal transduction. Lipid rafts also occur in embryonic stem (ES) cells, but their role in ES cells is largely unknown. Therefore, we investigated the role of lipid rafts in the maintenance of ES cell self-renewal. In the present study, we observed that the presence of lipid rafts/caveolae. The results from sucrose gradient fractionation showed that the expression of glycoprotein 130 (gp130) and leukemia inhibitory factor receptor beta (LIFRbeta) was decreased by treatment with methyl-beta-cyclodextrin (Mbeta-CD) but, interestingly, was not affected by caveolin-1 small interfering RNA (siRNA). In addition, LIF increased phosphorylation of signal tr

SUBMITTER: Lee MY 

PROVIDER: S-EPMC2903794 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature