Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells.

ABSTRACT: PURPOSE. To describe how developing amacrine cells and retinal ganglion cells (RGCs) differ in survival signaling and global gene expression. METHODS. Amacrine cells were immunopurified and processed for gene microarray analysis. For survival studies, purified amacrine cells were cultured at low density in serum-free medium, with and without peptide trophic factors and survival pathway inhibitors. The differences in gene expression between amacrine cells and RGCs were analyzed by comparing the transcriptomes of these two cell types at the same developmental ages. RESULTS. The amacrine cell transcriptome was very dynamic during development. Amacrine cell gene expression was remarkably similar to that of RGCs, but differed in several gene ontologies, including polarity- and neurotransmission

SUBMITTER: Kunzevitzky NJ 

PROVIDER: S-EPMC2904021 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature