Novel approaches to global mining of aberrantly methylated promoter sites in squamous head and neck cancer.

ABSTRACT:

Objective

Promoter hypermethylation is emerging as a promising molecular strategy for early detection of cancer. We examined promoter methylation status of 1143 cancer-associated genes to perform a global but unbiased inspection of methylated regions in head and neck squamous cell carcinoma (HNSCC).

Study design

Laboratory-based study.

Setting

Integrated health care system.

Subjects and methods

Five samples, two frozen primary HNSCC biopsies and three HNSCC cell lines, were examined. Whole genomic DNA was interrogated using a combination of DNA immunoprecipitation (IP) and Affymetrix whole-genome tiling arrays.

Results

Of the 1143 unique cancer genes on the array, 265 were recorded across five samples. Of the 265 genes, 55 were present in all five sample

SUBMITTER: Worsham MJ 

PROVIDER: S-EPMC2904310 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature