Metabolic profiling strategy for discovery of nutritional biomarkers: proline betaine as a marker of citrus consumption.

ABSTRACT:

Background

New food biomarkers are needed to objectively evaluate the effect of diet on health and to check adherence to dietary recommendations and healthy eating patterns.

Objective

We developed a strategy for food biomarker discovery, which combined nutritional intervention with metabolic phenotyping and biomarker validation in a large-scale epidemiologic study.

Design

We administered a standardized diet to 8 individuals and established a putative urinary biomarker of fruit consumption by using (1)H nuclear magnetic resonance (NMR) spectroscopic profiling. The origin of the biomarker was confirmed by using targeted NMR spectroscopy of various fruit. Excretion kinetics of the biomarker were measured. The biomarker was validated by using urinary NMR spectra from UK p

SUBMITTER: Heinzmann SS 

PROVIDER: S-EPMC2904656 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature