Hematopoietic differentiation: a coordinated dynamical process towards attractor stable states.

ABSTRACT:

Background

The differentiation process, proceeding from stem cells towards the different committed cell types, can be considered as a trajectory towards an attractor of a dynamical process. This view, taking into consideration the transcriptome and miRNome dynamics considered as a whole, instead of looking at few 'master genes' driving the system, offers a novel perspective on this phenomenon. We investigated the 'differentiation trajectories' of the hematopoietic system considering a genome-wide scenario.

Results

We developed serum-free liquid suspension unilineage cultures of cord blood (CB) CD34+ hematopoietic progenitor cells through erythroid (E), megakaryocytic (MK), granulocytic (G) and monocytic (Mo) pathways. These cultures recapitulate physiological hematopoiesis,

SUBMITTER: Felli N 

PROVIDER: S-EPMC2904736 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature