Aggregation of deamidated human betaB2-crystallin and incomplete rescue by alpha-crystallin chaperone.

ABSTRACT: Aging of the lens is accompanied by extensive deamidation of the lens specific proteins, the crystallins. Deamidated crystallins are increased in the insoluble proteins and may contribute to cataracts. Deamidation has been shown in vitro to alter the structure and decrease the stability of human lens betaB1, betaB2 and betaA3-crystallin. Of particular interest, betaB2 mutants were constructed to mimic the effect of in vivo deamidations at the interacting interface between domains, at Q70 in the N terminal domain and at Q162, its C-terminal homologue. The double mutant was also constructed. We previously reported that deamidation at the critical interface sites decreased stability, while preserving the dimeric 3D structure. In the present study, dynamic light scattering, differential scanni

SUBMITTER: Michiel M 

PROVIDER: S-EPMC2904749 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature