A three-state multi-ion kinetic model for conduction properties of ClC-0 chloride channel.

ABSTRACT: A three-state, multiion kinetic model was proposed to enable the conduction properties of the mammalian channel ClC-0 to be well characterized. Using this rate-theory based model, the current-voltage and conductance-concentration relations were obtained. The five parameters needed were determined by fitting the data of conduction experiments of the wild-type ClC-0 and its K519C mutant. The model was then tested against available calculation and simulation data, and the energy differences between distinct chloride-occupancy states computed agreed with an independent calculation on the binding free energies solved by using the Poisson-Boltzmann equation. The average ion number of conduction and the ion passing duration calculated closely resembled the values obtained from Brownian dynamics s

SUBMITTER: Wang XQ 

PROVIDER: S-EPMC2905080 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature