Tissue-specific transcriptome profiling of the citrus fruit epidermis and subepidermis using laser capture microdissection.

ABSTRACT: Most studies of the biochemical and regulatory pathways that are associated with, and control, fruit expansion and ripening are based on homogenized bulk tissues, and do not take into consideration the multiplicity of different cell types from which the analytes, be they transcripts, proteins or metabolites, are extracted. Consequently, potentially valuable spatial information is lost and the lower abundance cellular components that are expressed only in certain cell types can be diluted below the level of detection. In this study, laser microdissection (LMD) was used to isolate epidermal and subepidermal cells from green, expanding Citrus clementina fruit and their transcriptomes were compared using a 20k citrus cDNA microarray and quantitative real-time PCR. The results show striking dif

SUBMITTER: Matas AJ 

PROVIDER: S-EPMC2905196 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature