Adiponectin deficiency limits tumor vascularization in the MMTV-PyV-mT mouse model of mammary cancer.

ABSTRACT:

Purpose

High levels of the fat-secreted cytokine adiponectin (APN) are present in the circulation of healthy people, whereas low levels correlate with an increased incidence of breast cancer in women. The current study experimentally probes the physiologic functions of APN in mammary cancer in a newly generated genetic mouse model.

Experimental design

We established an APN null mouse model of mammary cancer by introducing the polyoma virus middle T (PyV-mT) oncogene expressed from mouse mammary tumor virus (MMTV) regulatory elements into APN null mice. MMTV-PyV-mT-induced tumors resemble ErbB2-amplified human breast cancers. We monitored tumor onset, kinetics, and animal survival, and analyzed vascular coverage, apoptosis, and hypoxia in sections from the primary tumors. Met

SUBMITTER: Denzel MS 

PROVIDER: S-EPMC2905461 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature