Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.

ABSTRACT: Quorum sensing (QS) cell-cell communication systems are utilized by bacteria to coordinate their behaviour according to cell density. Several different types of QS signal molecules have been identified, among which acyl-homoserine lactones (AHLs) produced by Proteobacteria have been studied to the greatest extent. Although QS has been studied extensively in cultured microorganisms, little is known about the QS systems of uncultured microorganisms and the roles of these systems in microbial communities. To extend our knowledge of QS systems and to better understand the signalling that takes place in the natural environment, metagenomic libraries constructed using DNA from activated sludge and soil were screened, using an Agrobacterium biosensor strain, for novel QS synthase genes. Three cos

SUBMITTER: Hao Y 

PROVIDER: S-EPMC2905786 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature