Alternative chemistries for the synthesis of thrombospondin-1 type 1 repeats.

ABSTRACT: Synthetic protein engineering has benefited from the development of diverse methods for the synthesis of functionalized peptide fragments and approaches for their subsequent assembly into full length polypeptides. Here we describe a series of synthetic approaches for the total chemical synthesis of the second type 1 repeat of thrombospondin-1 (TSR2) that vary in both the location of the ligation site and alpha-amine protecting group strategy (Boc/Fmoc) used for the synthesis of the associated peptide fragments. These syntheses illustrate that challenging peptide sequences can result from the protecting group strategy as well as from sequence-dependent factors. Importantly, we find that such challenges can be overcome by altering the chemistry used for solid phase peptide synthesis, the cho

SUBMITTER: Tiefenbrunn TK 

PROVIDER: S-EPMC2906232 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature