The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei.

ABSTRACT:

Background

Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins (VAPs) and candidate receptor proteins involved in WSSV infection, allow a better understanding of how these proteins interact in the viral life cycle. In this study, our aim was to find some host cellular membrane proteins that could bind with white spot syndrome virus (WSSV).

Results

Two proteins were evident by using a virus overlay protein binding assay (VOPBA) with WSSV. A protein with molecular weight 53 kDa, named BP53, was analyzed in this study, which was homologous with the F1-ATP synthase beta subunit by mass spectrometry analysis. Rapid amplification of cDNA ends (RACE) PCR was performed to identify the ful

SUBMITTER: Liang Y 

PROVIDER: S-EPMC2906456 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature