Isolation, characterization, and stability of discretely-sized nanolipoprotein particles assembled with apolipophorin-III.

ABSTRACT:

Background

Nanolipoprotein particles (NLPs) are discoidal, nanometer-sized particles comprised of self-assembled phospholipid membranes and apolipoproteins. NLPs assembled with human apolipoproteins have been used for myriad biotechnology applications, including membrane protein solubilization, drug delivery, and diagnostic imaging. To expand the repertoire of lipoproteins for these applications, insect apolipophorin-III (apoLp-III) was evaluated for the ability to form discretely-sized, homogeneous, and stable NLPs.

Methodology

Four NLP populations distinct with regards to particle diameters (ranging in size from 10 nm to >25 nm) and lipid-to-apoLp-III ratios were readily isolated to high purity by size exclusion chromatography. Remodeling of the purified NLP species over t

SUBMITTER: Fischer NO 

PROVIDER: S-EPMC2906516 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature