HIV Rev response element (RRE) directs assembly of the Rev homooligomer into discrete asymmetric complexes.

ABSTRACT: RNA is a crucial structural component of many ribonucleoprotein (RNP) complexes, including the ribosome, spliceosome, and signal recognition particle, but the role of RNA in guiding complex formation is only beginning to be explored. In the case of HIV, viral replication requires assembly of an RNP composed of the Rev protein homooligomer and the Rev response element (RRE) RNA to mediate nuclear export of unspliced viral mRNAs. Assembly of the functional Rev-RRE complex proceeds by cooperative oligomerization of Rev on the RRE scaffold and utilizes both protein-protein and protein-RNA interactions to organize complexes with high specificity. The structures of the Rev protein and a peptide-RNA complex are known, but the complete RNP is not, making it unclear to what extent RNA defines the c

SUBMITTER: Daugherty MD 

PROVIDER: S-EPMC2906596 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature