The tumour-suppressive miR-29a/b1 cluster is regulated by CEBPA and blocked in human AML.

ABSTRACT:

Background

CCAAT/enhancer-binding protein-alpha (CEBPA) is crucial for normal granulopoiesis and is frequently disrupted in acute myeloid leukaemia (AML). Increasing evidence suggests that CEBPA exerts its effects, in parts, by regulating specific microRNAs (miRNAs), as previously shown for miR-223. The aim of this study was to investigate the genome-wide pattern of miRNAs regulated by CEBPA in myeloid cells.

Methods

In Kasumi-1 cells, conditionally expressing CEBPA, we assessed the expression of 470 human miRNAs by microarray analysis. We further investigated the microarray results by qRT-PCR, luciferase reporter assays, and chromatin immunoprecipitation assays.

Results

In all, 18 miRNAs were more than two-fold suppressed or induced after CEBPA restoration. Among the

SUBMITTER: Eyholzer M 

PROVIDER: S-EPMC2906742 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature