Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells.

ABSTRACT:

Background

Parthenogenetic embryonic stem cells (PESCs) may have future utilities in cell replacement therapies since they are closely related to the female from which the activated oocyte was obtained. Furthermore, the avoidance of parthenogenetic development in mammals provides the most compelling rationale for the evolution of genomic imprinting, and the biological process of parthenogenesis raises complex issues regarding differential gene expression.

Methods and results

We describe here homozygous rhesus monkey PESCs derived from a spontaneously duplicated, haploid oocyte genome. Since the effect of homozygosity on PESCs pluripotency and differentiation potential is unknown, we assessed the similarities and differences in pluripotency markers and developmental potential

SUBMITTER: Sritanaudomchai H 

PROVIDER: S-EPMC2907230 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature