Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines.

ABSTRACT:

Background

Fascin induces membrane protrusions and cell motility. Fascin overexpression was associated with poor prognosis, and its downregulation reduces cell motility and invasiveness in esophageal squamous cell carcinoma (ESCC). Using a stable knockdown cell line, we revealed the effect of fascin on cell growth, cell adhesion and tumor formation.

Methods

We examined whether fascin is a potential target in ESCC using in vitro and in vivo studies utilizing a specific siRNA. We established a stable transfectant with downregulated fascin from KYSE170 cell line.

Results

The fascin downregulated cell lines showed a slower growth pattern by 40.3% (p < 0.01) and detachment from collagen-coated plates by 53.6% (p < 0.01), compared to mock cells, suggesting that fascin plays

SUBMITTER: Ortiz CM 

PROVIDER: S-EPMC2907320 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature