Requirement of ATM-dependent pathway for the repair of a subset of DNA double strand breaks created by restriction endonucleases.

ABSTRACT:

Background

DNA double strand breaks induced by DNA damaging agents, such ionizing radiation, are repaired by multiple DNA repair pathways including non-homologous end-joining (NHEJ) repair and homologous recombination (HR) repair. ATM-dependent DNA damage checkpoint regulates a part of DNA repair pathways, however, the exact role of ATM activity remains to be elucidated. In order to define the molecular structure of DNA double strand breaks requiring ATM activity we examined repair of DNA double strand breaks induced by different restriction endonucleases in normal human diploid cells treated with or without ATM-specific inhibitor.

Results

Synchronized G1 cells were treated with various restriction endonucleases. DNA double strand breaks were detected by the foci of phosphor

SUBMITTER: Suzuki K 

PROVIDER: S-EPMC2907562 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature