Comparison of next generation sequencing technologies for transcriptome characterization.

ABSTRACT:

Background

We have developed a simulation approach to help determine the optimal mixture of sequencing methods for most complete and cost effective transcriptome sequencing. We compared simulation results for traditional capillary sequencing with "Next Generation" (NG) ultra high-throughput technologies. The simulation model was parameterized using mappings of 130,000 cDNA sequence reads to the Arabidopsis genome (NCBI Accession SRA008180.19). We also generated 454-GS20 sequences and de novo assemblies for the basal eudicot California poppy (Eschscholzia californica) and the magnoliid avocado (Persea americana) using a variety of methods for cDNA synthesis.

Results

The Arabidopsis reads tagged more than 15,000 genes, including new splice variants and extended UTR regions. Of

SUBMITTER: Wall PK 

PROVIDER: S-EPMC2907694 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature