A gammaherpesvirus complement regulatory protein promotes initiation of infection by activation of protein kinase Akt/PKB.

ABSTRACT:

Background

Viruses have evolved to evade the host's complement system. The open reading frames 4 (ORF4) of gammaherpesviruses encode homologs of regulators of complement activation (RCA) proteins, which inhibit complement activation at the level of C3 and C4 deposition. Besides complement regulation, these proteins are involved in heparan sulfate and glycosaminoglycan binding, and in case of MHV-68, also in viral DNA synthesis in macrophages.

Methodology/principal findings

Here, we made use of MHV-68 to study the role of ORF4 during infection of fibroblasts. While attachment and penetration of virions lacking the RCA protein were not affected, we observed a delayed delivery of the viral genome to the nucleus of infected cells. Analysis of the phosphorylation status of a vari

SUBMITTER: Steer B 

PROVIDER: S-EPMC2908122 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature