Large-scale evidence for conservation of NMD candidature across mammals.

ABSTRACT:

Background

Alternatively-spliced (AS) forms can vary protein function, intracellular localization and post-translational modifications. AS coupled with mRNA nonsense-mediated decay (NMD) can also control the transcript abundance. Here, we have investigated the genome-scale conservation of alternatively-spliced NMD candidates (AS-NMD candidates), in mammals.

Methodology/principal findings

We mapped>12 million cDNA/EST library transcripts, comprising pooled data from both older and next-generation sequencing techniques, against genomic sequences to annotate AS-NMD candidates generated by in-frame premature termination codons (PTCs), in the human, mouse, rat and cow genomes. In these genomes, we found populations of genes that harbour AS-NMD candidates, varying in number from a

SUBMITTER: de Lima Morais DA 

PROVIDER: S-EPMC2908137 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature