Development of a Bacillus subtilis expression system using the improved Pglv promoter.

ABSTRACT:

Background

B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter Pglv. The system demonstrated high-level expression for target proteins in B. subtilis when induced by maltose. However, the system was markedly repressed by glucose. This limited the application of the system as a high-expression tool in biotechnology field. The aim of this study was to further improve the Pglv promoter system and enhance its expression strength.

Results

Here, site-directed mutagenesis was facilitated to enhance the expression strength

SUBMITTER: Ming YM 

PROVIDER: S-EPMC2908567 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature