The ketogenic diet suppresses the cathepsin E expression induced by kainic acid in the rat brain.

ABSTRACT:

Purpose

The ketogenic diet has long been used to treat epilepsy, but its mechanism is not yet clearly understood. To explore the potential mechanism, we analyzed the changes in gene expression induced by the ketogenic diet in the rat kainic acid (KA) epilepsy model.

Materials and methods

KA-administered rats were fed the ketogenic diet or a normal diet for 4 weeks, and microarray analysis was performed with their brain tissues. The effects of the ketogenic diet on cathepsin E messenger ribonucleic acid (mRNA) expression were analyzed in KA-administered and normal saline-administered groups with semi-quantitative and real-time reverse transcription polymerase chain reaction (RT-PCR). Brain tissues were dissected into 8 regions to compare differential effects of the ketogenic

SUBMITTER: Jeong HJ 

PROVIDER: S-EPMC2908849 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature