NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor.

ABSTRACT: Termination of translation in eukaryotes is triggered by two polypeptide chain release factors, eukaryotic class 1 polypeptide chain release factor (eRF1) and eukaryotic class 2 polypeptide chain release factor 3. eRF1 is a three-domain protein that interacts with eukaryotic class 2 polypeptide chain release factor 3 via its C-terminal domain (C-domain). The high-resolution NMR structure of the human C-domain (residues 277-437) has been determined in solution. The overall fold and the structure of the beta-strand core of the protein in solution are similar to those found in the crystal structure. The structure of the minidomain (residues 329-372), which was ill-defined in the crystal structure, has been determined in solution. The protein backbone dynamics, studied using (15)N-relaxation e

SUBMITTER: Mantsyzov AB 

PROVIDER: S-EPMC2909394 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature