Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells.

ABSTRACT: Coordination of cellular processes through the establishment of tissue-specific gene expression programs is essential for lineage maturation. The basic helix-loop-helix hemopoietic transcriptional regulator TAL1 (formerly SCL) is required for terminal differentiation of red blood cells. To gain insight into TAL1 function and mechanisms of action in erythropoiesis, we performed ChIP-sequencing and gene expression analyses from primary fetal liver erythroid cells. We show that TAL1 coordinates expression of genes in most known red cell-specific processes. The majority of TAL1's genomic targets require direct DNA-binding activity. However, one-fifth of TAL1's target sequences, mainly among those showing high affinity for TAL1, can recruit the factor independently of its DNA binding activity.

SUBMITTER: Kassouf MT 

PROVIDER: S-EPMC2909570 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature