Proteomic analysis of purified coronavirus infectious bronchitis virus particles.

ABSTRACT:

Background

Infectious bronchitis virus (IBV) is the coronavirus of domestic chickens causing major economic losses to the poultry industry. Because of the complexity of the IBV life cycle and the small number of viral structural proteins, important virus-host relationships likely remain to be discovered. Toward this goal, we performed two-dimensional gel electrophoresis fractionation coupled to mass spectrometry identification approaches to perform a comprehensive proteomic analysis of purified IBV particles.

Results

Apart from the virus-encoded structural proteins, we detected 60 host proteins in the purified virions which can be grouped into several functional categories including intracellular trafficking proteins (20%), molecular chaperone (18%), macromolcular biosynthes

SUBMITTER: Kong Q 

PROVIDER: S-EPMC2909931 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature