Comparative analysis of copy number detection by whole-genome BAC and oligonucleotide array CGH.

ABSTRACT:

Background

Microarray-based comparative genomic hybridization (aCGH) is a powerful diagnostic tool for the detection of DNA copy number gains and losses associated with chromosome abnormalities, many of which are below the resolution of conventional chromosome analysis. It has been presumed that whole-genome oligonucleotide (oligo) arrays identify more clinically significant copy-number abnormalities than whole-genome bacterial artificial chromosome (BAC) arrays, yet this has not been systematically studied in a clinical diagnostic setting.

Results

To determine the difference in detection rate between similarly designed BAC and oligo arrays, we developed whole-genome BAC and oligonucleotide microarrays and validated them in a side-by-side comparison of 466 consecutive clinic

SUBMITTER: Neill NJ 

PROVIDER: S-EPMC2909945 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature