Novel intronic microRNA represses zebrafish myf5 promoter activity through silencing dickkopf-3 gene.

ABSTRACT: A strong, negative cis-element located at the first intron +502/+835 (I300) of zebrafish myf5 has been reported. To elucidate the molecular mechanism underlying this repression network, we microinjected zebrafish single-cell embryos with I300 RNA, resulting in the dramatic reduction of luciferase activity driven by the myf5 promoter. Within this I300 segment, we identified an intronic microRNA (miR-In300) located at +609/+632 and found that it was more highly expressed in the older mature somites than those newly formed, which negatively correlated with the distribution of zebrafish myf5 transcripts. We proved that miR-In300 suppressed the transcription of myf5 through abolishing myf5 promoter activity, and we subsequently identified the long isoform of the Dickkopf-3 gene (dkk3) as the ta

SUBMITTER: Hsu RJ 

PROVIDER: S-EPMC2910042 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature