Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples.

ABSTRACT: Next-generation sequencing has proven an extremely effective technology for molecular counting applications where the number of sequence reads provides a digital readout for RNA-seq, ChIP-seq, Tn-seq and other applications. The extremely large number of sequence reads that can be obtained per run permits the analysis of increasingly complex samples. For lower complexity samples, however, a point of diminishing returns is reached when the number of counts per sequence results in oversampling with no increase in data quality. A solution to making next-generation sequencing as efficient and affordable as possible involves assaying multiple samples in a single run. Here, we report the successful 96-plexing of complex pools of DNA barcoded yeast mutants and show that such 'Bar-seq' assessment o

SUBMITTER: Smith AM 

PROVIDER: S-EPMC2910071 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature