Global gene expression changes in rat retinal ganglion cells in experimental glaucoma.

ABSTRACT:

Purpose

Intraocular pressure (IOP) is an important risk factor in glaucoma. Gene expression changes were studied in glaucomatous rat retinal ganglion cells (RGCs) to elucidate altered transcriptional pathways.

Methods

RGCs were back-labeled with Fluorogold. Unilateral IOP elevation was produced by injection of hypertonic saline into the episcleral veins. Laser capture microdissection (LCM) was used to capture an equal number of RGCs from normal and glaucomatous retinal sections. RNA was extracted and amplified, labeled, and hybridized to rat genome microarrays, and data analysis was performed. After selected microarray data were confirmed by RT-qPCR and immunohistochemistry, biological pathway analyses were performed.

Results

Significant changes were found in the expr

SUBMITTER: Wang DY 

PROVIDER: S-EPMC2910641 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature