P120ctn and P-cadherin but not E-cadherin regulate cell motility and invasion of DU145 prostate cancer cells.

ABSTRACT:

Background

Adherens junctions consist of transmembrane cadherins, which interact intracellularly with p120ctn, beta-catenin and alpha-catenin. p120ctn is known to regulate cell-cell adhesion by increasing cadherin stability, but the effects of other adherens junction components on cell-cell adhesion have not been compared with that of p120ctn.

Methodology/principal findings

We show that depletion of p120ctn by small interfering RNA (siRNA) in DU145 prostate cancer and MCF10A breast epithelial cells reduces the expression levels of the adherens junction proteins, E-cadherin, P-cadherin, beta-catenin and alpha-catenin, and induces loss of cell-cell adhesion. p120ctn-depleted cells also have increased migration speed and invasion, which correlates with increased Rap1 but not Ra

SUBMITTER: Kumper S 

PROVIDER: S-EPMC2910720 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature