Induction of human beta-cell proliferation and engraftment using a single G1/S regulatory molecule, cdk6.

ABSTRACT:

Objective

Most knowledge on human beta-cell cycle control derives from immunoblots of whole human islets, mixtures of beta-cells and non-beta-cells. We explored the presence, subcellular localization, and function of five early G1/S phase molecules-cyclins D1-3 and cdk 4 and 6-in the adult human beta-cell.

Research design and methods

Immunocytochemistry for the five molecules and their relative abilities to drive human beta-cell replication were examined. Human beta-cell replication, cell death, and islet function in vivo were studied in the diabetic NOD-SCID mouse.

Results

Human beta-cells contain easily detectable cdks 4 and 6 and cyclin D3 but variable cyclin D1. Cyclin D2 was only marginally detectable. All five were principally cytoplasmic, not nuclear. Overexpre

SUBMITTER: Fiaschi-Taesch NM 

PROVIDER: S-EPMC2911074 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature