Dynamic repositioning of dorsal to two different kappaB motifs controls its autoregulation during immune response in Drosophila.

ABSTRACT: Autoregulation is one of the mechanisms of imparting feedback control on gene expression. Positive autoregulatory feedback results in induction of a gene, and negative feedback leads to its suppression. Here, we report an interesting mechanism of autoregulation operating on Drosophila Rel gene dorsal that can activate as well as repress its expression. Using biochemical and genetic approaches, we show that upon immune challenge Dorsal regulates its activation as well as repression by dynamically binding to two different kappaB motifs, kappaB(I) (intronic kappaB) and kappaB(P) (promoter kappaB), present in the dorsal gene. Although the kappaB(I) motif functions as an enhancer, the kappaB(P) motif acts as a transcriptional repressor. Interestingly, Dorsal binding to these two motifs is dynam

SUBMITTER: Mrinal N 

PROVIDER: S-EPMC2911332 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature