Intranasal M cell uptake of nanoparticles is independently influenced by targeting ligands and buffer ionic strength.

ABSTRACT: In mucosal tissues, epithelial M cells capture and transport microbes across the barrier to underlying immune cells. Previous studies suggested that high affinity ligands targeting M cells may be used to deliver mucosal vaccines; here, we show that particle composition and dispersion buffer ionic strength can independently influence their uptake in vivo. First, addition of a poloxamer 188 to nanoparticle formulations increased uptake of intranasally administered nanoparticles in vivo, but the effect was dependent on the presence of the M cell-targeting ligand. Second, solvent ionic strength is known to effect electrostatic interactions; accordingly, reduced ionic strength increased the electrostatic potential between the epithelium and the particles. Interestingly, below a critical ionic s

SUBMITTER: Rajapaksa TE 

PROVIDER: S-EPMC2911333 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature