Unlocking short read sequencing for metagenomics.

ABSTRACT:

Background

Different high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved.

Methodology/principal findings

We describe an experimental and computational pipeline yielding millions of reads that can exceed 200 bp with quality scores approaching that of traditional Sanger sequencing. The method combines an automatable gel-less library construction step with paired-end sequencing on a short-read instrument. With appropriately sized library inserts, mate-pair sequences can overlap, and we describe the SHERA software package that joins them to form a longer composite read.

Conclusions/significance

This strategy is broad

SUBMITTER: Rodrigue S 

PROVIDER: S-EPMC2911387 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature