Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig.

ABSTRACT:

Background

Several chronic conditions leading to skeletal muscle dysfunction are known to be associated with changes in the expression of myosin heavy chain (MHC) isoforms at both the mRNA and protein level. Many of these conditions are modelled, pre-clinically, in the guinea pig due to similar disease onset and progression to the human condition, and their generally well-characterised anatomy. MHC composition is amenable to determination by protein and mRNA based methodologies, the latter quantifying the expression of MHC isoform-specific gene transcripts allowing the detection of earlier, and more subtle changes. As such, the MHC mRNAs, and specific oligonucleotide primers of all common laboratory species have been available for some time. However, due to incomplete genomic annot

SUBMITTER: Tonge DP 

PROVIDER: S-EPMC2911452 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature