Semi-automated Sholl analysis for quantifying changes in growth and differentiation of neurons and glia.

ABSTRACT: There is a need to develop therapies that promote growth or remyelination of mammalian CNS axons. Although the feasibility of pre-clinical treatment strategies should be tested in animal models, in vitro assays are usually faster and less expensive. As a result, in vitro models are ideal for screening large numbers of potential therapeutics prior to use in more complex in vivo systems. In 1953, Sholl introduced a technique that is a reliable and sensitive method for quantifying indices of neurite outgrowth. However, application of the technique is limited because it is labor-intensive. Several methods have been developed to reduce the analysis time for the Sholl technique; but these methods require extensive pre-processing of digital images, they introduce user bias or they have not been c

SUBMITTER: Gensel JC 

PROVIDER: S-EPMC2911480 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature